Coding
Part:BBa_K5492402:Design
Designed by: Norbert Nyeste, Sandor Ban, Vojtech Vasut, Sara Simonka Group: iGEM24_TERMOSZ-Selye-HUN (2024-09-15)
Wild type HNMT - pETDUET-1
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 129
Illegal EcoRI site found at 235
Illegal XbaI site found at 47 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 129
Illegal EcoRI site found at 235 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 129
Illegal EcoRI site found at 235
Illegal BglII site found at 1015
Illegal BamHI site found at 123
Illegal XhoI site found at 457
Illegal XhoI site found at 1064 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 129
Illegal EcoRI site found at 235
Illegal XbaI site found at 47 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 129
Illegal EcoRI site found at 235
Illegal XbaI site found at 47
Illegal NgoMIV site found at 1034 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Restriction sites adapted to pETDUET-1, with adjustions also added for proper framing of the gene.
Source
https://www.ncbi.nlm.nih.gov/nuccore/AH003685, BBa_K5492400, BBa_K5492100